1-Fluoro-2,4-dinitrobenzene (CAS 70-34-8)

1-Fluoro-2,4-dinitrobenzene is a chemical compound frequently used in biochemical research as a reagent for labeling the nucleophilic amino acids in proteins, particularly cysteine and histidine. This compound reacts selectively with thiol and imidazole groups in proteins, forming a covalent bond, which makes it a useful tool for studying protein structure, function, and protein-protein interactions. In addition, 1-Fluoro-2,4-dinitrobenzene is employed in the quantification of these nucleophilic amino acids within various protein samples. The compound′s reactivity is also exploited in synthetic chemistry to introduce the 2,4-dinitrophenyl (DNP) moiety into other molecules. In proteomics and enzymology, 1-Fluoro-2,4-dinitrobenzene aids researchers in probing the active sites of enzymes and in elucidating mechanisms of enzyme action.

References:

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