Anti-hIFN-γ Neutralizing mAb

Neutralizing IgA monoclonal antibody to human interferon-gamma

Neutralizing monoclonal antibody against human IFN-γ

Anti-hIFN-γ-hIgA2 (clone H7WM120) is a recombinant monoclonal antibody (mAb) able to neutralize human interferon-gamma (hIFN-γ). This mAb is expressed and produced in chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. Thereby, common hybridoma-related drawbacks such as the generation of non-relevant mAbs containing aberrant light chains are avoided [1]. 

Anti-hIFN-γ-hIgA2 consists of the variable region of the Anti-hIFN-γ mAb (clone H7WM120) and the engineered human IgA2 constant region. This antibody has been selected for its ability to efficiently neutralize the biological activity of hIFN-γ. It was found to have significantly higher neutralizing activity than its corresponding IgG isotype. 

Key features:

• Potent and specific neutralization activity against hIFN-γ
• Free from non-relevant mAbs found in hybridoma-based productions
• Provided azide-free
• Each lot is functionally tested

IFN-γ plays a role in activating lymphocytes to enhance anti-microbial and anti-tumor effects [2-4].

 More details

References:

1. Bradbury A. et al. 2018. When monoclonal antibodies are not monospecific: Hybridomas frequently express additional functional variable regions. mAbs, 10(4), 539–546.
2. Ivashkiv L.B., 2018. IFNγ: signalling, epigenetics and roles in immunity, metabolism, disease and cancer immunotherapy. Nat Rev Immunol. 18(9):545-558.
3. Shtrichman R. & Samuel CE., 2001. The role of gamma interferon in antimicrobial immunity. Curr Opin Microbiol. 4(3):251-9.
4. Sato A. et al., 2006. Antitumor activity of IFN-lambda in murine tumor models. J Immunol. 176(12):7686-94.

Figures

Anti-hIFN-γ-IgA is a potent neutralization antibody. Increasing concentrations of Anti-hIFN-γ-IgA were incubated for 30 minutes with the recombinant cytokine hIFN-γ (0.3 ng/ml) prior to the addition of HEK-Blue™ IFN-γ cells. After overnight incubation, SEAP activity in the cell culture supernatant was assessed using QUANTI-Blue™ Solution. Data are shown in percentage (%) of neutralization.

Specifications

Target: Human interferon-gamma (hIFN-γ)

Source: CHO cells

Clone: H7WM120

Isotype: Human IgA2, kappa

Control: Human IgA2 Control

Purification: Affinity chromatography with peptide M

Formulation: 0.2 µm filtered solution in Tris HCl buffer with saccharose, glycine, and stabilizing agents

Applications: Blocking & Neutralization

Quality control:

• This product have been validated using neutralization cellular assays.
• The complete sequence of the antibody has been verified.
• The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.

Contents

• 200 μg purified anti-hIFNγ-IgA antibody, provided azide-free and lyophilized.

 The product is shipped at room temperature.

 Store lyophilized antibody at -20 °C.

 Lyophilized product is stable for at least 1 year.

 Avoid repeated freeze-thaw cycles.

Details

Interferon-gamma (IFN-γ), a Type II interferon, is secreted from CD4+ T-helper 1 (Th1) cells and activated natural killer (NK) cells. It plays a role in activating lymphocytes to enhance anti-microbial and anti-tumor effects [1-3]. In addition, IFN-γ plays a role in regulating the proliferation, differentiation, and response of lymphocyte subsets. 

IFN-γ exerts its action by first binding to a heterodimeric receptor consisting of two chains, IFNGR1 and IFNGR2, causing its dimerization and the activation of specific Janus family kinases (JAK1 and JAK2) [4, 5]. Two STAT1 molecules then associate with this ligand-activated receptor complex and are activated by phosphorylation. Activated STAT1 forms homodimers and are translocated to the nucleus where they bind interferon-gamma-activated sites (GAS) in the promoter of IFN-γ inducible genes.

1. Ivashkiv L.B., 2018. IFNγ: signalling, epigenetics and roles in immunity, metabolism, disease and cancer immunotherapy. Nat Rev Immunol. 18(9):545-558.
2. Shtrichman R. & Samuel CE., 2001. The role of gamma interferon in antimicrobial immunity. Curr Opin Microbiol. 4(3):251-9.
3. Sato A. et al., 2006. Antitumor activity of IFN-lambda in murine tumor models. J Immunol. 176(12):7686-94.
4. Platanias L.C., 2005. Mechanisms of type-I- and type-II-interferon-mediated signalling. Nat Rev Immunol. 5(5):375-86.
5. Schroder K. et al., 2004. Interferon-gamma: an overview of signals, mechanisms, and functions. J Leukoc Biol. 75(2):163-89.

Citations